Product Pathways - Development
DLL3 (G93) Antibody #2483
|2483S||100 µl (10 western blots)||---||In Stock||---|
|2483||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Mouse.
Specificity / Sensitivity
DLL3 (G93) Antibody detects transfected levels of DLL3. It does not recognize transfected levels of rat DLL1 and human DLL4.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding residue Gly93 of mouse DLL3. Antibodies are purified by protein A and peptide affinity chromatography.
Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).
Mutations in DLL3 cause Spondylocostal dysostoses (SCD), a diverse group of disorders of axial skeletal malformation (7-10).
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- Chiba, S. (2006) Stem Cells 24, 2437-2447.
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- Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
- LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.
- Whittock, N.V. et al. (2004) Clin. Genet. 66, 67-72.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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