Product Class: Other

MspI Methyltransferase
NEBU cloned at NEB recombinant 37 No SAM

The L size of this product has been discontinued on 11/15/16. The S size will still be available.

M0215MspI_thumb

Product Introduction

MspI Methyltransferase recognizes the same sequence as HpaII Methyltransferase, but modifies the external cytosine residue (C5) of the sequence CCGG.

Catalog # Size Concentration
M0215S 100.0 units 5000 units/ml

Product Information

Description

MspI Methyltransferase recognizes the same sequence as the HpaII Methyltransferase, but modifies the external cytosine residue (C5) of the sequence CCGG.

Product Source

An E. coli strain that carries the cloned MspI modification gene from Moraxella species (ATCC 49670).
This product is related to the following categories:
Methyltransferases for Epigenetics Products,
Methyltransferases Products,
This product can be used in the following applications:
DNA Methylation Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by MspI restriction endonuclease.

Reaction Conditions

1X MspI Methylase Reaction Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 37°C

1X MspI Methylase Reaction Buffer
50 mM Tris-HCl
100 mM NaCl
5 mM β-ME
10 mM EDTA
(pH 7.5 @ 25°C)

Control Protection Assay

MspI Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X MspI Methyltransferase Reaction Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by MspI Methyltransferase is determined by the addition of 40 µl NEBuffer 2 supplemented with 10 mM MgCl2 and 5 units of MspI restriction endonuclease. Incubation for MspI at 37°C is followed by analysis on agarose gels.

Storage Buffer

50 mM Tris-HCl
50 mM NaCl
5 mM β-ME
10 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 40397 daltons

References

  1. Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.

Protocols, Manuals & Usage

Protocols

  1. Recommended Protocol for 3H labeling of DNA
  2. Recommended Protocol for Methylation of Genomic DNA

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
  2. What should be considered if the methylation is not going to completion?
  3. What is the molecular weight of MspI Methyltransferase?
  4. Will any NEB methyltransferases (methylases) work on RNA?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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