Product Introduction

• Double-stranded DNA specific exonuclease
• Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases
• Initiates at nicked sites in double-stranded DNA
• Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction
• Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease III is ideal for:

• Site-directed mutagenesis
• Preparation of single-stranded DNA for dideoxy sequencing
• Preparation of nested deletions in double-stranded DNA