DescriptionAnti-CBD Monoclonal Antibody is a murine anti-chitin binding domain (CBD) antibody, isotype IgG1.
Properties and Usage
IsotypeMouse Isotype IgG1
Quality Assurance Statement
- In an ELISA assay, a dilution of 1/1,000 gives a signal of at least 20% of the maximum signal using high concentrations of antibody. The same dilution gives a strong signal when used to detect chitin-binding domain fusion protein in Western blots developed with the Phototope-HRP Western Blot Detection System (Cell Signaling Technology). This antibody does not cross-react with other E. coli proteins.
- Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM NaN3 or an equivalent antimicrobial agent.
- Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., Pelletier, J.J., Paulus, H. and Xu, M.-Q. (1997). Gene. 192, 271-281.
- Watanabe, T., Ito, Y., Yamada, T., Hashimoto, M., Sekine, S. and Tanaka, H. (1994). J. Bacteriol. 176, 4465-4472.