DescriptionAnti-CBD Monoclonal Antibody is a murine anti-chitin binding domain (CBD) antibody, isotype IgG1.
Properties and Usage
IsotypeMouse Isotype IgG1
Quality Assurance Statement
- In an ELISA assay, a dilution of 1/1,000 gives a signal of at least 20% of the maximum signal using high concentrations of antibody. The same dilution gives a strong signal when used to detect chitin-binding domain fusion protein in Western blots developed with the Phototope-HRP Western Blot Detection System (Cell Signaling Technology). This antibody does not cross-react with other E. coli proteins.
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
- Physical Purity (HPLC):
The purity of the product is determined by HPLC analysis.
- Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM NaN3 or an equivalent antimicrobial agent.
- Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., Pelletier, J.J., Paulus, H. and Xu, M.-Q. (1997). Gene. 192, 271-281.
- Watanabe, T., Ito, Y., Yamada, T., Hashimoto, M., Sekine, S. and Tanaka, H. (1994). J. Bacteriol. 176, 4465-4472.