Product Class: NEBNext

NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

Now supplied with a sample of NEBNext High-Fidelity 2X PCR Master Mix
Catalog #SizeConcentration
E6200S12 reactions
E6200L60 reactions


The NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing of Chromatin Immunoprecipitated DNA. Each of these components must pass rigorous quality control standards and are lot Controlled, both individually and as a set of reagents.

ChIP DNA Library Preparation Workflow for Illumina

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Phosphorylation Reaction Buffer-2010X
Deoxynucleotide Solution Mix10 mM
T4 DNA Polymerase
DNA Polymerase I, Large (Klenow) Fragment-20
T4 Polynucleotide Kinase
Deoxyadenosine 5´- Triphosphate (dATP)-201 mM
Klenow Fragment (3´→5´ exo_)
NEBuffer 2 for Klenow Fragment (3´→ 5´ exo)10X
Quick T4 DNA Ligase
Quick Ligation Reaction Buffer2X
Phusion High-Fidelity PCR Master Mix with HF Buffer2X
NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)2X

Properties and Usage

Storage Temperature


Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].


  1. For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact for further information.
  1. Protocol (E6200) for Use With NEBNext Singleplex (#E7350) or Multiplex Oligos for Illumina (#E7335, #E7500)
  2. Protocol (E6200) for Use With End User Supplied Primers and Adaptors