DescriptionThe NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing of Chromatin Immunoprecipitated DNA. Each of these components must pass rigorous quality control standards and are lot Controlled, both individually and as a set of reagents.
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
|Phosphorylation Reaction Buffer||10X|
|Deoxynucleotide Solution Mix||10 mM|
|T4 DNA Polymerase|
|DNA Polymerase I, Large (Klenow) Fragment|
|T4 Polynucleotide Kinase|
|Deoxyadenosine 5´- Triphosphate (dATP)||1 mM|
|Klenow Fragment (3´→5´ exo_)|
|NEBuffer 2 for Klenow Fragment (3´→ 5´ exo–)||10X|
|Quick T4 DNA Ligase|
|Quick Ligation Reaction Buffer||2X|
|Phusion High-Fidelity PCR Master Mix with HF Buffer||2X|
|NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)||2X|
Properties and Usage
Legal and Disclaimers* Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion is a registered trademark and property of Thermo Fisher Scientific.Illumina is a registered trademark of Illumina, Inc.
Material Safety Datasheets
- NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® MSDS
- Phosphorylation Reaction Buffer MSDS
- Deoxynucleotide Solution Mix MSDS
- T4 DNA Polymerase MSDS
- DNA Polymerase I, Large (Klenow) Fragment MSDS
- T4 Polynucleotide Kinase MSDS
- Deoxyadenosine 5´- Triphosphate (dATP) MSDS
- NEBuffer 2 for Klenow Fragment (3´→ 5´ exo–) MSDS
- Quick Ligation Reaction Buffer MSDS
- Phusion High-Fidelity PCR Master Mix with HF Buffer MSDS
- NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE) MSDS
- For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.