- Transformation efficiency: 0.6-1 x 109 cfu/μg pUC19 DNA
- Enhanced BL21 derivative
- Methionine auxotroph (metB1) designed for seleno-methionine labeling of proteins
- Derivative of T7 Express with T7 RNA Polymerase on the chromosome under lac control
- Deficient in proteases Lon and OmpT
- Resistant to T1 phage (fhuA2)
- Free of animal products
- T7 expression
- Protease deficient
- SeMet labeling for protein crystallography
GenotypefhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 metB1 Δ(mcrC-mrr)114::IS10
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|pUC19 Transformation Control Plasmid||-20||0.05 ng/μl|
|SOC Outgrowth Medium||4||1X|
Advantages and Features
Properties and Usage
|Antibiotics for Plasmid Selection||Working Concentration|
- Ships on dry ice
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
- Transformation Efficiency:
The competent cells are tested for transformation efficiency and pass minimum release criteria. Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 μg of plasmid into a given volume of competent cells.
Legal and Disclaimers
Research Use Only
- Notice to Buyer/User:The buyer and user have a nonexclusive license to use this system or any component thereof for RESEARCH PURPOSES ONLY. See Assurance Letter and Statement attached hereto for details on terms of the license granted hereunder.
Material Safety Datasheets
- CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
- STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
- Lambert, A. R. et al. (2008). Structure. 16, 558-569.
- Sambrook, J. et al. (1989). Cold Spring Habor Laboratory Press. Molecular Cloning: A Laboratory Manual . (2nd ed.), (Appendix A.3)
- Why are there no colonies or no growth in liquid culture (C3022)?
- Why is there no protein visible on gel or no activity (C3022)?
- Why is induced protein insoluble (C3022)?
- What are the solutions/recipes (C3022)?
- What are the strain properties (C3022)?
- Can I store competent cells at -20°C instead of -80°C?
- What is the difference between NEB #C3022H and NEB #C3022I?
- What is the optimal heat shock time for this strain (NEB #C3022H and NEB #C3022I)?
- How long should I incubate DNA with cells on ice for this strain (NEB #C3022H and NEB #C3022I)?
- Which kind of transformation tubes should be used?
- What volume of DNA can be added into competent cells?
- What is the shelf life for this strain (NEB #C3022H and NEB #C3022I)?
- Are NEB's competent cells compatible with the "Plate and Go" protocol?