Product Class: Other

NEBExpress® Competent E. coli (High Efficiency)

Having trouble opening your tubes? NEB Tube Opener can help!

Product Introduction

High efficiency chemically competent E. coli cells suitable for non-T7 protein expression.

  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Free of animal products
  • No dry ice surcharge on competent cell shipments
Catalog # Size Concentration
C2523H 20.0 x 0.05 ml
C2523I 6.0 x 0.2 ml

Protocols, Manuals & Usage

Protocols

  1. High Efficiency Transformation (C2523)
  2. 5 Minute Transformation (C2523)
  3. Expression Using NEB Express (C2523)
  4. High Efficiency Transformation Protocol (NEB #C2523 and #C3037)

Usage & Guidelines

Application Notes

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
  2. How long should I incubate cells on ice after DNA has been added (NEB #C2523H and NEB #C2523I)?
  3. What are the solutions/recipes (C2523)?
  4. What are the strain properties (C2523)?
  5. What is the difference between NEB #C2523H and NEB #C2523I?
  6. Why are there no colonies or no growth in liquid culture (C2523)?
  7. Why is induced protein insoluble (C2523)?
  8. Why is there no protein visible on gel or no activity (C2523)?
  9. Can I store competent cells at -20°C instead of -80°C?
  10. Can the NEBExpress Competent E.coli (High Efficiency) be used for the expression of constructs containing a T7 promoter?
  11. What is the optimal heat shock time for this strain (NEB #C2523H and NEB #C2523I)?
  12. Which kind of transformation tubes should be used?
  13. What volume of DNA can be added into competent cells?
  14. What is the shelf life for this strain (NEB #C2523H and NEB #C2323I)?
  15. Are NEB's competent cells compatible with the “Mix & Go" protocol?
  16. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?

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