Product Pathways - Adhesion
α-N-Catenin Antibody #2131
|2131S||100 µl (10 western blots)||---||In Stock||---|
|2131||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||102||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
α-N-Catenin Antibody detects endogenous levels of α-N-catenin protein. The antibody does not cross-react with other α-catenin family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding the carboxy-terminus of human α-N-catenin. Antibodies are purified using protein A and peptide affinity chromatography.
Western blot analysis of extracts from SHSY5Y and PC12 cells, using α-N-Catenin Antibody.
Confocal immunofluorescent analysis of SH-SY5Y cells using α-N-Catenin Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
Flow cytometric analysis of PC-12 cells, using α-N-Catenin antibody (blue) compared to a nonspecific negative control antibody (red).
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).
Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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