Product Pathways - Jak/Stat Pathway
Stat3 (79D7) Rabbit mAb (Sepharose® Bead Conjugate) #4368
|4368S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|4368||carrier free and custom formulation / quantity||email request|
|IP||1:20||Human, Mouse, Rat, Monkey||Endogenous||79, 86||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IP=Immunoprecipitation
Specificity / Sensitivity
Stat3 (79D7) Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total Stat3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein.
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Stat3 (79D7) Rabbit mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation assay of Stat3 proteins.
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
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- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.