His-Tag (27E8) Mouse mAb (Sepharose® Bead Conjugate) #4079
|4079S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|4079||carrier free and custom formulation / quantity||email request|
|IP||1:20||Transfected Only||Mouse IgG1|
Applications Key: IP=Immunoprecipitation
Specificity / Sensitivity
His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide.
Immunoprecipitation of extracts from COS cells transfected with a construct overexpressing Tyro3-His using His-Tag (27E8) Mouse mAb (Sepharose® bead conjugate) (lane 1). Mouse IgG Isotype Control (Sepharose® bead conjugate) #3420 (lane 2) was used as a negative control. The western blot was probed using His-Tag Antibody #2365.
This Cell Signaling Technology (CST) antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. His-Tag (27E8) Mouse mAb (Sepharose® Bead Conjugate) is useful for immunoprecipitation assays.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.