Product Pathways - Stem Cell and Lineage Markers
Sox2 (C70B1) Rabbit mAb (IHC Preferred) #3728
|3728S||100 µl (250 sections)||---||In Stock||---|
|3728||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Sox2 (C70B1) Rabbit mAb (IHC Preferred) detects endogenous levels of Sox2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences at the carboxy-terminal region of mouse Sox2.
Western blot analysis of extracts from mouse embryonic stem cells (mESCs) and F9 cells using Sox2 (C70B1) Rabbit mAb (IHC Preferred).
Immunohistochemical analysis of paraffin-embedded F9 cells (positive, left) and C2C12 cells (negative, right) using Sox2 (C70B1) Rabbit mAb (IHC Preferred).
Immunohistochemical analysis of paraffin-embedded 14.5 day mouse embryo using Sox2 (C70B1) Rabbit mAb (IHC Preferred). Note staining of ventricular/subventricular zone.
Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal (1). Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state (2,3). Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development (4,5). siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (6). Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state (7,8). Additional evidence demonstrates that Sox2 is also present in adult multipotent progenitors that give rise to some adult epithelial tissues, including several glands, the glandular stomach, testes, and cervix. Sox2 is thought to regulate target gene expression important for survival and regeneration of these tissues (9).
- Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.
- Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
- Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
- Boyer, L.A. et al. (2005) Cell 122, 947-56.
- Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.
- Matin, M.M. et al. (2004) Stem Cells 22, 659-68.
- Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
- Okita, K. et al. (2007) Nature 448, 313-7.
- Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.