Product Pathways - Development
Sox2 (D6D9) XP® Rabbit mAb #3579
|3579S||100 µl (10 western blots)||---||In Stock||---|
|3579P||40 µl (4 western blots)||---||In Stock||---|
|3579||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog, Horse.
Specificity / Sensitivity
Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of Sox2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2.
Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus using Sox2 (D6D9) XP (TM) Rabbit mAb.
Immunohistochemical anaylsis of paraffin-embedded NTERA2 cells (positive) (left) and HeLa cells (negative) (right) using Sox2 (D6D9) XP® Rabbit mAb.
Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).
- Nichols, J. et al. (1998) Cell 95, 379-391.
- Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
- Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
- Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
- Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
- Boyer, L.A. et al. (2005) Cell 122, 947-956.
- Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.
- Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.
- Kregel, S. et al. (2013) PLoS One 8, e53701. Applications: IHC-P (paraffin), Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.