Product Pathways - Cell Cycle / Checkpoint
Phospho-NPM (Thr95) Antibody #3517
|3517S||100 µl (10 western blots)||---||In Stock||---|
|3517||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey.
Specificity / Sensitivity
Phospho-NPM (Thr95) Antibody detects endogenous levels of NPM only when phosphorylated at Thr95.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr95 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts of HeLa cells, untreated or nocodazole-treated, using Phospho-NPM (Thr95) Antibody (upper) or NPM Antibody #3542 (lower).
Confocal immunofluorescent analysis of HeLa cells untreated (left) and λ phosphatase-treated (right), using NPM (Thr95) phosphate Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells using Phospho-NPM (Thr95) Antibody versus propidium iodide (DNA content). The box indicates phospho-NPM positive cells.
Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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