Product Pathways - PathScan ELISA
PathScan® Total GFP Sandwich ELISA Antibody Pair #7879
|7879S||1 Kit (Reagents for 4 x 96 well plates)||---||In Stock||---|
|7879||carrier free and custom formulation / quantity||email request|
|Kit Includes||Volume||Cap Color|
|GFP Capture Ab (100X)||0.4 ml||Pink|
|GFP Detection Ab (100X)||0.4 ml||Blue|
|Anti-mouse IgG, HRP-linked Antibody||0.04 ml||Yellow|
Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Important Ordering Details
Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at firstname.lastname@example.org.
CST's PathScan® Total GFP Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total GFP Sandwich ELISA Kit #7878. This antibody pair is intended for use with cotransfected GFP as a convenient means of monitoring transfection efficiency and may not detect GFP fusion proteins. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The GFP rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysate is added followed by a GFP mouse detection antibody and HRP-conjugated, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of GFP.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm (1). Among other applications, GFP is commonly cotransfected alongside a gene of interest as a means of monitoring transfection efficiency.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 9998 BSA
- 9803 Cell Lysis Buffer (10X)
- 2956 GFP (D5.1) XP® Rabbit mAb
- 2555 GFP Antibody
- 7878 PathScan® Total GFP Sandwich ELISA Kit
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween® 20 (PBST-20X)
- 7002 STOP Solution
- 7004 TMB Substrate
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
PathScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.