Product Pathways - Neuroscience
Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody #4027
|4027S||100 µl (10 western blots)||---||In Stock||---|
|4027||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Human, Mouse.
Specificity / Sensitivity
Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody detects endogenous levels of GluR 2 only when phosphorylated at Tyr876. It may also detect GluR 3 when phosphorylated at the conserved Tyr887. This residue is not conserved in GluR 1 or GluR 4.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr876 of human GluR 2. Antibodies are purified by protein A and peptide affinity chromatography.
AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).
Src family tyrosine kinases phosphorylate the GluR 2 subunit of AMPA receptors at Tyr876, which increases the interaction with GRIP1/2 but not PICK1. In addition, Tyr876 is important for AMPA- and NMDA-induced GluR 2 internalization (3).
The phosphorylation site at Tyr876 was also independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of GluR at Tyr876 was observed in extracts isolated from ischemic rat brain.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 5306 AMPA Receptor (GluR 2) (D39F2) Rabbit mAb
- 13607 AMPA Receptor (GluR 2) (E1L8U) Rabbit mAb
- 2460 AMPA Receptor (GluR 2/3/4) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 3362 CaMKII (pan) Antibody
- 4782 PKA C-α Antibody
- 2507 PSD95 Antibody
- 3356 Phospho-CaMKII (Tyr231) Antibody
- 3381 Phospho-NMDAR1 (Ser890) Antibody
- 4206 Phospho-NMDAR2A (Tyr1246) Antibody
- 4781 Phospho-PKA C (Thr197) Antibody
- 9921 Phospho-PKC Antibody Sampler Kit
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 2503 Stargazin Antibody
- 4234 nNOS Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.