Product Pathways - Development
β-Catenin Antibody Sampler Kit #2951
|2951S||1 Kit (5 x 40 µl)||---||In Stock||---|
|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|β-Catenin (D10A8) XP® Rabbit mAb #8480||40 µl||W, IP, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP||H, M, R, Mk||Z, B, Pg, GP, Hr||92||Rabbit IgG|
|Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561||40 µl||W||H, M, R, Mk||C, X, Z, B, Dg, Pg||92||Rabbit|
|Phospho-β-Catenin (Thr41/Ser45) Antibody #9565||40 µl||W||H, M, Mk||Dg, Pg||92||Rabbit|
|Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651||40 µl||W, IP||H||M, R, X, Z||92||Rabbit IgG|
|Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176||40 µl||W, IP, IF-F, IF-IC||H||M, R, C, X, Z||92||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561.
Western blot analysis of extracts from 293 cells pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Thr41/Ser45) Antibody #9565.
Western blot analysis of extracts from MKN-45 and SK-N-MC cells, untreated or treated with λ phosphatase for 1 hour or forskolin (FSK) for 30 minutes, using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176(upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).
Western blot analysis of extracts from SK-N-MC and NTERA-2 cells, untreated or λ phosphatase-treated, using Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651 (upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).
The β-Catenin Antibody Sampler Kit provides an economical means of detecting total β-catenin as well as β-catenin phosphorlylated at various residues. The kit contains enough primary and secondary antibody to perform four Western blots with each antibody.
Specificity / Sensitivity
Each antibody in this kit recognizes only its specific target and does not cross-react with other family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human β-catenin or residues surrounding Thr41 and Ser45 of human β-catenin. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein, a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin protein, or a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin.
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
Both Akt and PKA were shown to phosphorylate β-catenin at Ser552 and Ser675. Phosphorylation at Ser552 and Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-12).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
- Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976.
- Fang, D. et al. (2007) J. Biol. Chem. 282, 11221-11229.
- He, X.C. et al. (2007) Nat. Genet. 39, 189-198.
- Hino, S. et al. (2005) Mol. Cell Biol. 25, 9063-9072.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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