Product Pathways - Chromatin Regulation / Epigenetics
SirT1 Antibody (Mouse Specific) #2028
|2028S||100 µl (10 western blots)||---||In Stock||---|
|2028||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SirT1 Antibody (Mouse Specific) detects endogenous levels of total SirT1 protein. This antibody does not cross-react with other sirtuin proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse SirT1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from F9, C2C12 and PYS-2 cells using SirT1 Antibody (Mouse Specific).
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined (9).
- Guarente, L. (1999) Nat. Genet. 23, 281-285.
- Vaziri, H. et al. (2001) Cell 107, 149-159.
- Luo, J. et al. (2001) Cell 107, 137-148.
- Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
- Brunet, A. et al. (2004) Science 303, 2011-2015.
- Motta, M.C. et al. (2004) Cell 116, 551-563.
- Picard, F. et al. (2004) Nature 429, 771-776.
- Rodgers, J.T. et al. (2005) Nature 434, 113-118.
- Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.
- Jeong, H. et al. (2011) Nat Med 18, 159-65. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2525 Acetyl-p53 (Lys382) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 2880 FoxO1 (C29H4) Rabbit mAb
- 2497 FoxO3a (75D8) Rabbit mAb
- 9472 FoxO4 Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 2627 SirT3 (C73E3) Rabbit mAb
- 2590 SirT6 Antibody
- 2527 p53 (7F5) Rabbit mAb
- 9282 p53 Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.