Product Pathways - Apoptosis
Caspase-6 Antibody #9762
|9762S||100 µl (10 western blots)||---||In Stock||---|
|9762||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||15, 35||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35 kDa) and the small subunit of caspase-6 resulting from cleavage at aspartic acid 193 (15 kDa). This antibody does not recognize other caspases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of caspase-6. Antibodies are purified by protein A and peptide affinity chromatography.
Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.
- Hara, H. et al. (2002) J. Immunol. 168, 2288-2295. Applications: Western Blotting.
- Schroder, A. et al. (2002) . J. Immunol. 168, 996-1000. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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