Product Pathways - Apoptosis
Caspase-3 (3G2) Mouse mAb #9668
|9668S||100 µl (10 western blots)||---||In Stock||---|
|9668T||20 µl (2 western blots)||---||In Stock||---|
|9668||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human||Endogenous||17, 19, 35||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Caspase-3 (3G2) Mouse mAb detects endogenous levels of full length (35 kDa) and the large fragment (17/19 kDa) of caspase-3 resulting from cleavage at aspartic acid 175.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids 28-44 of human caspase-3.
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
- Ikner, A. and Ashkenazi, A. (2011) J Biol Chem 286, 21546-54. Applications: Western Blotting.
- Gucký, T. et al. (2013) J Med Chem 56, 6234-47. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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