Product Pathways - Apoptosis
PARP Antibody #9542
|9542L||300 µl (30 western blots)||---||In Stock||---|
|9542S||100 µl (10 western blots)||---||In Stock||---|
|9542P||40 µl (4 western blots)||---||In Stock||---|
|9542||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||89, 116||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
PARP Antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage. The antibody does not cross-react with related proteins or other PARP isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the caspase cleavage site in PARP. Antibodies are purified by protein A and peptide affinity chromatography.
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
- Li, J. et al. (2002) . J. Biol. Chem. 277, 388-394. Applications: Western Blotting.
- Cheong, J. W. et al. (2003) Clin Cancer Res. 9 (13), 5018-27. Applications: Western Blotting.
- Soldatenkov, V. A. et al. (2002) . J. Biol. Chem. 277, 665-670. Applications: IP.
- Chandrasekar, B. et al. (2004) J. Biol. Chem. 279, 20221-20233. Applications: Western Blotting.
- Kumar-Sinha, C. et al. (2003) Cancer Res. 63, 132-139. Applications: Western Blotting.
- Biton, S. and Ashkenazi, A. (2011) Cell 145, 92-103. Applications: Western Blotting.
- Jak, M. et al. (2011) Blood 118, 5178-88. Applications: Western Blotting.
- May, K.L. et al. (2010) Infect Immun 78, 4691-6. Applications: Western Blotting.
- He, S. et al. (2009) Cell 137, 1100-11. Applications: Western Blotting.
- Thome, C.H. et al. (2012) Mol Cell Proteomics , . Applications: Western Blotting.
- Elzi, D.J. et al. (2012) Mol Cell Biol 32, 4388-99. Applications: Western Blotting.
- Gupta, S. et al. (2013) PLoS Pathog 9, e1003272. Applications: Western Blotting.
- Iden, S. et al. (2012) Cancer Cell 22, 389-403. Applications: Western Blotting.
- Hung, T. et al. (2011) Nat Genet 43, 621-9. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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