Product Pathways - PI3K / Akt Signaling
Phospho-GSK-3β (Ser9) Antibody #9336
|9336L||300 µl (30 western blots)||---||In Stock||---|
|9336S||100 µl (10 western blots)||---||In Stock||---|
|9336||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||46||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Zebrafish, Bovine.
Specificity / Sensitivity
Phospho-GSK-3beta (Ser9) Antibody detects endogenous levels of GSK-3β only when phosphorylated at serine 9. The antibody may cross-react weakly with the phosphorylated form of GSK-3α due to high sequence homology.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of human GSK-3β. Antibodies are purified by protein A and peptide affinity chromatography.
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Crowder, R.J. and Freeman, R.S. (2000) J. Biol. Chem. 275, 34266-34271. Applications: Western Blotting.
- Fasshauer, M. et al. (2000) J. Biol. Chem. 275, 25494-25501. Applications: Western Blotting.
- Ohteki, T. et al. (2000) J. Exp. Med. 192, 991-104. Applications: Western Blotting.
- Okano, J. et al. (2000) J. Biol. Chem. 275, 30934-30942. Applications: Western Blotting.
- Nakamura, N. et al. (2000) Mol. Cell. Biol. 20, 8969-8982. Applications: Western Blotting.
- Morfini, G. et al. (2002) EMBO 21 (3), 281-293. Applications: IC-IF.
- Vitari, A.C. et al. (2004) Biochem J 378, 257-68. Applications: Western Blotting.
- Hirai, S. et al. (2006) J Neurosci 26, 11992-2002. Applications: Western Blotting.
- Fujita, H. et al. (2005) Biochem Pharmacol 69, 1773-84. Applications: Western Blotting.
- Guertin, D.A. et al. (2009) Cancer Cell 15, 148-59. Applications: Western Blotting.
- Kubota, H. et al. (2012) Mol Cell 46, 820-32. Applications: Western Blotting.
- Jinesh, G.G. et al. (2012) Cell Death Differ , . Applications: Western Blotting.
- Green, H.F. and Nolan, Y.M. (2012) Transl Psychiatry 2, e194. Applications: Western Blotting.
- Green, H.F. and Nolan, Y.M. (2012) Neurochem Int 61, 666-71. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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