Product Pathways - MAPK Signaling
MEK1/2 Antibody #9122
|9122L||300 µl (30 western blots)||---||In Stock||---|
|9122S||100 µl (10 western blots)||---||In Stock||---|
|9122||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey, D. melanogaster||Endogenous||45||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Chicken, Xenopus.
Specificity / Sensitivity
MEK1/2 Antibody detects endogenous levels of total MEK1/2 protein. The antibody does not cross-react with related kinases such as SEK (MKK4), MKK3 or MKK6.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MEK1/2. Antibodies are purified by protein A and peptide affinity chromatography.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting.
- Thompson, T. et al. (2010) Mol Cancer Ther 9, 1158-68. Applications: Western Blotting.
- Piatelli, M. J. et al. (2004) J. Immunol. 172, 2753-2762. Applications: Western Blotting.
- Umenishi, F. and Schrier, R.W. (2003) J. Biol. Chem. 278, 15765-15770. Applications: Western Blotting.
- Rosenberger, C.M. and Finlay, B.B. (2002) . The Journal of Biological Chemistry 277, 18753-18762. Applications: Western Blotting.
- Deszo, E. L. et al. (2001) . Journal of Biological Chemistry 276, 10212-10217. Applications: Western Blotting.
- Furuno, T. et al. (2001) . Journal of Immunology 166, 4416-4421. Applications: IC-IF, Western Blotting.
- Bueno, O. F. et al. (2000) . European Molecular Biology Organization (EMBO) 19, 6341-6350. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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