Product Pathways - MAPK Signaling
Phospho-MEK1/2 (Ser217/221) Antibody #9121
|9121L||300 µl (30 western blots)||---||In Stock||---|
|9121S||100 µl (10 western blots)||---||In Stock||---|
|9121||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey, S. cerevisiae||Endogenous||45||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Chicken.
Specificity / Sensitivity
Phospho-MEK1/2 (Ser217/221) Antibody detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. This antibody does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2. Antibodies are purified by protein A and peptide affinity chromatography.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
CST's Phospho- MEK1/2 (Ser217/221) Antibody selectively recognizes active MEK, i.e., only when phosphorylated at Ser217/221, and hence is an excellent marker of MEK1/2 activity.
- Piatelli, M.J. et al. (2004) J. Immunol. 172, 2753-2762. Applications: Western Blotting.
- Umenishi, F. and Schrier, R.W. (2003) J. Biol. Chem. 278, 15765-15770. Applications: Western Blotting.
- Rosenberger, C.M. et al. (2002) . The Journal of Biological Chemistry 277, 18753-18762. Applications: Western Blotting.
- MacNicol, M.C. et al. (2000) . J. Biol. Chem. 275, 3803-3809. Applications: Western Blotting.
- Pizon, V. et al. (2000) . Oncogene 19, 6074-6081. Applications: Western Blotting.
- Wang, X. et al. (2000) . J. Biol. Chem. 275, 39435-39443. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 9146 MEK1 (30C8) Rabbit mAb
- 2352 MEK1 (61B12) Mouse mAb
- 9124 MEK1 Antibody
- 9126 MEK1/2 (47E6) Rabbit mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
- 9122 MEK1/2 Antibody
- 9160 MEK1/2 HeLa Control Cell Extracts
- 9147 MEK2 (13E3) Rabbit mAb
- 9125 MEK2 Antibody
- 9128 Phospho-MEK1 (Ser298) Antibody
- 9127 Phospho-MEK1 (Thr286) Antibody
- 9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
- 2338 Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
- 9120 PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 9903 U0126
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.