Product Pathways - Lymphocyte Signaling
Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb #9115
|9115S||100 µl (10 western blots)||---||In Stock||---|
|9115||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse||Endogenous||95-100||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb detects endogenous levels of total Blimp-1/PRDI-BF1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human PRDI-BF1 isoform 2.
Western blot analysis of extracts from Karpas 620, SR and H929 cell lines using Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb.
Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B-cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T-cells (4,5) suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13).
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- Smith, M.A. et al. (2010) J Immunol 185, 6058-67. Applications: Chromatin IP, Western Blotting.
- Desai, S. et al. (2010) Mol Cancer Res 8, 907-18. Applications: Western Blotting.
- Cubedo, E. et al. (2011) FEBS J 278, 3065-75. Applications: Chromatin IP.
- Kikuchi, H. et al. (2014) J Leukoc Biol 95, 399-404. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
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