Product Class: Other

Monarch® DNase I, Lyophilized


Catalog #T2104

Product Introduction

  • Lyophilized form of DNase I
  • Stable at room temperature for up to 3 years prior to rehydration
  • RNase-free formulation
  • DNase I reaction buffer included
  • Ideal for removal of contaminating genomic DNA from RNA samples
  • Included as a component in Monarch kits, also offered individually for convenience

Product Information

Description

Monarch DNase I, Lyophilized is an RNase-free, lyophilized format of DNA specific Endonuclease, DNase I, supplied in a dry cake. It is also included as a component of the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110). After rehydration with nuclease-free water, the resulting enzyme solution is suitable for preparing DNase reactions with the supplied reaction buffer. Optimal DNA removal is achieved either on-column or in-solution. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids. 
This product is related to the following categories:
Total RNA Extraction & Purification,
DNA Modifying Enzymes & Cloning Technologies,
Nucleic Acid Purification,
This product can be used in the following applications:
Nucleic Acid Purification,
Cloning & Synthetic Biology

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • T2104S     25    
      Monarch® DNase I, Lyophilized T2104-1 25 1 x 2,200 units Not Applicable
      Monarch® DNase I Reaction Buffer T2105-1 25 1 x 4.6 ml Not Applicable

Properties & Usage

Materials Required but not Supplied

Protocols, Manuals & Usage

Protocols

  1. Protocol for Rehydration of Monarch DNase I, Lyophilized (NEB #T2104)
  2. Protocol for DNase digestion reaction in solution using Monarch DNase I, Lyophilized (NEB #T2104)
  3. Protocol for on-column DNase reaction using Monarch DNase I, Lyophilized (NEB #T2104) and Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)

FAQs & Troubleshooting

FAQs

  1. What is the difference between Monarch DNase I, Lyophilized (NEB #T2104) and DNase I, (RNase-free) (NEB #M0303)?
  2. How do I store the Monarch DNase I, Lyophilized (NEB #T2104) upon receiving it?
  3. How do I store Monarch DNase I, Lyophilized (NEB #T2104) after rehydration?
  4. How many freeze-thaws can the rehydrated Monarch DNase I, Lyophilized (NEB #T2104) tolerate?
  5. What volume should I rehydrate in to use with Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)?
  6. What volume should I rehydrate in to make it equivalent to NEB’s liquid DNase product NEB #M0303?
  7. I want a concentrated form of DNase that is higher than the typical range. What is the minimum volume for rehydration of Monarch DNase I, Lyophilized (NEB #T2104)?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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