Magnetic mRNA Isolation KitProduct information
|25 isolations||-||Unavailable in your region|
DescriptionThe New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. Oligo d(T)25 Magnetic Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound dT DNA as a primer in a first-strand cDNA reaction.
- Suitable for automated high-throughput applications
- Eliminates need for phenol or other organic solvents
- No need to precipitate poly(A)+ transcripts in eluent
- Obtain intact poly(A)+ RNA in less than one hour
- Negligible gDNA contamination
- Reusable beads
- Oligo d(T)25 Magnetic Beads
- Lysis/Binding Buffer
- Low Salt Buffer
- Wash Buffer I
- Wash Buffer II
- Elution Buffer (S1560, S1550)
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Oligo d(T)25 Magnetic Beads||4||5 mg/ml|
|Low Salt Buffer||1X|
|Wash Buffer I||1X|
|Wash Buffer II||1X|
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Oligo d(T)25 Magnetic Beads
Low Salt Buffer
Wash Buffer I
Wash Buffer II
- Wu, N. et al. (2004). Mol. Biotech.. 27, 119-125.