Random Primer 9Product information
Random Primer 9
|1.0 A260 units||-||Unavailable in your region|
A mixture of random nonanucleotides used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). The primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabeled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N9) primer ensures the presence of virtually all sequence combination of nanomer primers which results in equally labelled DNA of high specific activity (1,2).
Oligolabeling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).
5' d(N9) 3' [N = A,C,G,T]
Sequence5' d(N9) 3' [N = A,C,G,T]
- Product Categories:
- RT-PCR Products,
- Reverse Transcriptases & RT-PCR Products,
- cDNA Synthesis & Reverse Transcriptases
Properties and Usage
- 12.4 nmol of S1254 are supplied.
- Feinberg, A.P. and Vogelstein, B. (1983). Anal. Biochem.. 132, 6-13.
- Feinberg, A.P. and Vogelstein, B. (1984). Anal. Biochem.. 137, 266-267.
- Grunstein, M. and Hogness, D.S. (1975). Proc. Natl. Acad. Sci. USA. 72, 3961-3965.
- Southern, E.M. (1975). J. Mol. Biol.. 98, 503-517.
- Smith, G.E. and Summers, M.D. (1980). Anal. Biochem.. 109, 123-129.
- Hasse, A. et al. (1984). Methods Virol.. 7, 189-226.
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Quality & Safety
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Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Random Primer 9
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