Product Class: Other

Random Primer 9

Product Introduction

A mixture of random nonanucleotides used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). The primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabeled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N9) primer ensures the presence of virtually all sequence combination of nanomer primers which results in equally labelled DNA of high specific activity (1,2).

Oligolabeling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).

Sequence

5' d(N9) 3' [N = A,C,G,T]

Catalog # Size Concentration
S1254S 1 A260 units 0 %

Product Information

Description

 

Sequence

5' d(N9) 3' [N = A,C,G,T]
Product Categories:
RT-PCR Products,
Reverse Transcriptases & RT-PCR Products,
cDNA Synthesis & Reverse Transcriptases
Applications:
RT-PCR & cDNA Synthesis,
RT-qPCR, RT-PCR and cDNA Synthesis,
RNA Modification
,
PCR

Properties and Usage

Storage Temperature

-20°C

Notes

  1. 12.4 nmol of S1254 are supplied.

References

  1. Feinberg, A.P. and Vogelstein, B. (1983). Anal. Biochem.. 132, 6-13.
  2. Feinberg, A.P. and Vogelstein, B. (1984). Anal. Biochem.. 137, 266-267.
  3. Grunstein, M. and Hogness, D.S. (1975). Proc. Natl. Acad. Sci. USA. 72, 3961-3965.
  4. Southern, E.M. (1975). J. Mol. Biol.. 98, 503-517.
  5. Smith, G.E. and Summers, M.D. (1980). Anal. Biochem.. 109, 123-129.
  6. Hasse, A. et al. (1984). Methods Virol.. 7, 189-226.

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Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

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