HpaII
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0171S |
HpaII, recombinant |
2.000 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0171L |
HpaII, recombinant |
10.000 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0171M |
HpaII, recombinant, conc. |
10.000 units ( 50000 units/ml ) | - | Unavailable in your region |
HpaII
HpaII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10232250. Learn more.
Product Introduction
- Time-Saver™ qualified for digestion in 5-15 minutes
- 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
- Methylation-sensitive restriction enzyme
- Restriction Enzyme Cut Site: C/CGG
Catalog # | Size | Concentration |
---|---|---|
R0171S | 2000 units | 10000 units/ml |
R0171L | 10000 units | 10000 units/ml |
R0171M | 10000 units | 50000 units/ml |
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- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
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Product Information
Description
Product Source
An E. coli strain that carries the HpaII gene from Haemophilus parainfluenzae (ATCC 49669).- This product is related to the following categories:
- Hydroxymethylation Detection and Analysis,
- Methylation Sensitive and Methylation Dependent Restriction Enzymes for Epigenetics,
- Restriction Enzymes for Epigenetic Analysis,
- DNA Methylation Analysis,
- Restriction Endonucleases H M,
- Time-Saver Qualified Restriction Enzymes,
- This product can be used in the following applications:
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
- Restriction Enzyme Digestion
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 37°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 100%NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: <10%
rCutSmart™ Buffer: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
80°C for 20 minutesMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked
Isoschizomers
BsiSI
HapII
MspI
Related Products
Materials Sold Separately
Product Notes
- HpaII is an isoschizomer of MspI.
- Inhibited by salt concentrations > 50 mM KCl.
- Blocked by CpG methylation.
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Site Preferences
- Star Activity
- Traditional Cloning Quick Guide
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Comparison of Methods for DNA Methylation Analysis
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5' Overhangs
- Restriction Enzymes for Epigenetics Selection Chart
- Time-Saver™ Qualified Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- Is HpaII inhibited by salt?
- Is HpaII affected by methylation?
- What is the molecular weight of HpaII?
- Does spermidine increase HpaII activity?
- Does HpaII cleave ssDNA?
- What is the activity of HpaII at 25°C?
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
- Are HpaII slow sites found in common vectors?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
Troubleshooting
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specification Change Notifications
HpaII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10232250. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0171M_v1_0621211
- R0171M_v1_0621301
- R0171M_v1_0621303
- R0171M_v1_0621310
- R0171S_L_v1_0621211
- R0171S_L_v1_0621301
- R0171S_L_v1_0621303
- R0171S_L_v1_0621307
- R0171S_L_v1_0621310
- R0171M_v1_0621403
- R0171S_L_v1_0621403
- R0171S_L_v1_0621408
- R0171S_L_v1_0621501
- R0171S_L_v1_0621409
- R0171S_L_v1_0621502
- R0171M_v1_0681507
- R0171S_L_v1_0681507
- R0171M_v1_0681601
- R0171S_L_v1_0681601
- R0171M_v1_0681606
- R0171S_L_v1_0681606
- R0171S_L_v1_0681612
- R0171M_v1_0681704
- R0171S_L_v1_0681704
- R0171M_v1_0681711
- R0171S_L_v1_0681709
- R0171S_L_v1_0681804
- R0171M_v1_10012452
- R0171L_v1_10022418
- R0171M_v1_10037267
- R0171S_v1_10037269
- R0171L_v1_10047381
- R0171M_v1_10047727
- R0171L_v1_10050636
- R0171S_v1_10056461
- R0171L_v1_10059952
- R0171S_v1_10059935
- R0171M_v1_10079010
- R0171S_v1_10076435
- R0171S_v1_10079952
- R0171L_v1_10086005
- R0171S_v1_10091189
- R0171L_v1_10090001
- R0171L_v1_10091694
- R0171M_v1_10091632
- R0171M_v1_10092115
- R0171S_v1_10091627
- R0171L_v1_10091631
- R0171M_v1_10104944
- R0171S_v1_10104943
- R0171L_v1_10112779
- R0171S_v1_10132208
- R0171L_v1_10138026
- R0171M_v1_10151008
- R0171L_v1_10151011
- R0171S_v1_10151009
- R0171M_v1_10172485
- R0171S_v1_10181591
- R0171L_v1_10181590
- R0171M_v1_10222995
- R0171S_v1_10223983
- R0171S_v1_10229439
- R0171M_v2_10232259
- R0171L_v2_10232258
- R0171S_v2_10232260
- R0171M_v2_10250720
- R0171S_v2_10255359
- R0171L_v2_10253172
- R0171L_v2_10274352
- R0171S_v2_10280504
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.HpaII
rCutSmart™ Buffer
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
InvitrogenLicensed Under U.S. Patent No. 5,248,605
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.