NEBExpress GamS Nuclease Inhibitor is a recombinant protein that inhibits Exonuclease V (RecBCD) activity and stabilizes linear DNA templates in E. coli based in vitro protein synthesis reactions.
Enhances synthesis yield of linear DNA using the NEBExpress Cell-free E. coli Protein Synthesis System
Recombinant enzyme; ≥ 95% purity, as determined by SDS-PAGE
No detectable protease activity, ensuring stability of desired target protein
Compatible with various target proteins, ranging from 17 to 230 kDa
Optimal storage buffer for performance in protein synthesis reactions
Flexible reaction conditions achieve maximum yield; activity can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures
NEBExpress GamS Nuclease Inhibitor is a recombinant protein that inhibits Exonuclease V (RecBCD) activity and stabilizes linear DNA templates in E. coli based in vitro protein synthesis reactions.
Figure 1: Protein synthesis of a linear DNA template using the NEBExpress® Cell-free E. coli Protein Synthesis System supplemented with NEBExpress GamS Nuclease Inhibitor
50 µl reactions containing 100 ng linear template DNA, the components of the NEBExpress® Cell-free E. coli Protein Synthesis System and 1.5 µg NEBExpress GamS Nuclease Inhibitor incubated for 5 hours at 37°C were monitored for activity as determined by fluorescence signal.
Product Source
Cloned from bacteriophage lambda and expressed in E. coli.
This product is related to the following categories:
The NEBExpress Cell-free E. coli Protein Synthesis System is compatible with linear DNA templates. To achieve expression levels comparable to plasmid DNA templates, include 1.5 µg NEBExpress GamS Nuclease Inhibitor per 50 µL reaction containing 250 ng template DNA.
A detailed product manual for the NEBExpress Cell-free E. coli Protein Synthesis System, with protocols can be found here.
The typical incubation temperature is 37°C. Lowering the incubation temperature can help the folding of some target proteins. The reaction can be incubated at temperatures from 16°C to 42°C and protein synthesis will continue steadily for 10 hours at 37°C or up to 24 hours at lower temperatures, depending on DNA input and aeration.
The NEBExpress protein synthesis reaction can be scaled up; all reaction components, including the GamS, must be scaled proportionally.
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Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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Licenses
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us [email protected] While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications
