Product Class: Other

NEBExpress® GamS Nuclease Inhibitor
cloned at NEB recombinant 37


Catalog #P0774

Product Introduction

NEBExpress GamS Nuclease Inhibitor is a recombinant protein that inhibits Exonuclease V (RecBCD) activity and stabilizes linear DNA templates in E. coli based in vitro protein synthesis reactions.  

  • Enhances synthesis yield of linear DNA using the NEBExpress Cell-free E. coli Protein Synthesis System
  • Recombinant enzyme; ≥ 95% purity, as determined by SDS-PAGE 
  • No detectable protease activity, ensuring stability of desired target protein
  • Compatible with various target proteins, ranging from 17 to 230 kDa
  • Optimal storage buffer for performance in protein synthesis reactions
  • Flexible reaction conditions achieve maximum yield; activity can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures

Product Information

Description

NEBExpress GamS Nuclease Inhibitor is a recombinant protein that inhibits Exonuclease V (RecBCD) activity and stabilizes linear DNA templates in E. coli based in vitro protein synthesis reactions.  

Figure 1: Protein synthesis of a linear DNA template using the NEBExpress® Cell-free E. coli Protein Synthesis System supplemented with NEBExpress GamS Nuclease Inhibitor



50 µl reactions containing 100 ng linear template DNA, the components of the NEBExpress® Cell-free E. coli Protein Synthesis System and 1.5 µg NEBExpress GamS Nuclease Inhibitor incubated for 5 hours at 37°C were monitored for activity as determined by fluorescence signal.

Product Source

Cloned from bacteriophage lambda and expressed in E. coli.
 
This product is related to the following categories:
NEBExpress® Cell-free E. coli Protein Synthesis System,
Cell-Free Protein Expression,
Protein Expression,
This product can be used in the following applications:
NEBExpress® Cell-free E. coli Protein Synthesis System,
Cell-Free Protein Expression,
Protein Expression

Properties & Usage

Storage Buffer

50 mM Tris-acetate
pH 8.2

Features

  • Enhances synthesis yield of linear DNA using the NEBExpress Cell-free E. coli Protein Synthesis System
  • No detectable protease activity, ensuring stability of desired target protein
  • Compatible with various target proteins, ranging from 17 to 230 kDa
  • Flexible reaction conditions achieve maximum yield; activity can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures
 

Application Features

  • Expression of Linear DNA
  • Expression of difficult target proteins
  • Expression of proteins in high throughput workflows

Product Notes

  1. The NEBExpress Cell-free E. coli Protein Synthesis System is compatible with linear DNA templates. To achieve expression levels comparable to plasmid DNA templates, include 1.5 µg NEBExpress GamS Nuclease Inhibitor per 50 µL reaction containing 250 ng template DNA.
  2. A detailed product manual for the NEBExpress Cell-free E. coli Protein Synthesis System, with protocols can be found here. 
  3. The typical incubation temperature is 37°C. Lowering the incubation temperature can help the folding of some target proteins. The reaction can be incubated at temperatures from 16°C to 42°C and protein synthesis will continue steadily for 10 hours at 37°C or up to 24 hours at lower temperatures, depending on DNA input and aeration.
  4. The NEBExpress protein synthesis reaction can be scaled up; all reaction components, including the GamS, must be scaled proportionally. 

Protocols, Manuals & Usage

Protocols

  1. Typical Protocol for NEBExpress® GamS Nuclease Inhibitor when used with the NEBExpress® Cell-free E. coli Protein Synthesis System (NEB #E5360)

Application Notes

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. How does NEBExpress® GamS Nuclease Inhibitor enhance protein yield?
  2. How much linear DNA template and NEBExpress® GamS Nuclease Inhibitor should I add to my reaction?
  3. Is more NEBExpress® GamS Nuclease Inhibitor always better?
  4. Can I freeze-thaw NEBExpress®GamS Nuclease Inhibitor?
  5. How should I prepare my linear DNA?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us [email protected] While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications

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