Product Introduction

• Decaps RNA that contains ADP-containing, non-canonical initiating nucleotides (including NAD⁺/NADH caps), resulting in ligatible monophosphate at the 5′ end.
• Hydrolyzes NADH and NAD⁺ into AMP and reduced nicotinamide monophosphate nucleoside (NMNH), and AMP and nicotinamide monophosphate nucleoside (NMN⁺), respectively.
• Hydrolyzes diphosphate dinucleotides and metabolic co-factors containing an ADP moiety such as AppA, FAD, Coenzyme A, etc.
• Useful for NAD⁺ decapping or deNADding when NAD⁺ is the 5′ initiating nucleotide of RNA.
• Useful for detection and identification of RNA containing non-canonical initiating nucleotides using ligation-based methods such as 5′ RACE and RNA-seq.

This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.