Product Class: Other

Alkaline Phosphatase, Calf Intestinal (CIP)

New! Quick Dephosphorylation Kit offers heat inactivation, high specific activity and a fast protocol.

icon_CutSmart icon_incTemp37 icon_HI_no

Product Introduction

Alkaline Phosphatase, Calf Intestinal (CIP) nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters.

  • Faster reaction setup (no supplemental additives like zinc required)
  • Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
  • Less enzyme required (high specific activity), resulting in a lower cost per reaction
  • No need for multiple phosphatases (CIP removes 5´- and 3´- phosphates from DNA, RNA and dNTPs)
  • Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
 
Catalog # Size Concentration
M0290S 1,000 units 10,000 units/ml
M0290L 5,000 units 10,000 units/ml

Faqs & Tech Tips

FAQs

  1. Which alkaline phosphatase, Quick CIP, from the Quick Dephosphorylation Kit, rSAP, CIP or Antarctic Phosphatase works best?
  2. The number of colonies that do not contain an insert seems high, how can I tell if the CIP worked?
  3. Will CIP work in restriction enzyme NEBuffers, including CutSmart?
  4. Does the DNA need to be purified after the restriction digest, prior to CIP treatment?
  5. Can CIP be heat inactivated?
  6. Why do some protocols recommend using CIP at 50°C and some recommend 37°C?
  7. What phosphate groups are removed by rSAP, CIP, Quick CIP, or AnP?
  8. Does the DNA need to be purified after CIP treatment?
  9. Will Quick CIP, rSAP, CIP, or AnP dephosphorylate proteins?
  10. What is the effect of metal chelators, inorganic phosphate and phosphate analogs on CIP activity?”
  11. What is the effect of monovalent salts on CIP activity?
  12. What is the effect of reducing agents on CIP activity?
  13. Does the DNA need to be purified after a restriction digest and prior to the dephosphorylation step?
  14. Does the DNA need to be purified after the dephosphorylation step and prior to the ligation step?
  15. Are the alkaline phosphatases active in NEBuffers?
  16. Cloning Problem:  Too much background in the transformation step.
  17. Which alkaline phosphatase, rSAP, CIP, or Antarctic Phosphatase, works best?
  18. Which alkaline phosphatase, rSAP, CIP, or Antarctic Phosphatase, works best?

Protocols & Manuals

Protocols

  1. Protocol for Dephosphorylation of 5´-ends of DNA using CIP (NEB #M0290)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Selection Charts

Interactive Tools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.