Product Class: Other

Mung Bean Nuclease
NEBU 30

Product Introduction

  • Single-stranded (ssDNA or RNA) specific endonuclease
  • Catalyzes the removal of single-stranded extension in double-stranded DNA
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Mung Bean can be used for:

  • Removal of both 3' and 5' single-stranded overhangs from double-stranded DNA to create blunt ends
  • Cleavage of single-stranded DNA and RNA
  • Cleavage of the single-stranded region in a DNA hairpin
  • Mapping of RNA transcripts
Catalog # Size Concentration
M0250S 1500 units 10000 units/ml
M0250L 7500 units 10000 units/ml

Product Information

Description

 

Product Source

Mung bean sprouts.
This product is related to the following categories:
Exonucleases and Non-specific Endonucleases,
RNA Modification,
This product can be used in the following applications:
Blunting

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • M0250S     -20    
      Mung Bean Nuclease M0250SVIAL -20 1 x 0.15 ml 10,000 units/ml
      Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X
  • M0250L     -20    
      Mung Bean Nuclease M0250LVIAL -20 1 x 0.75 ml 10,000 units/ml
      Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of M13mp18 single-stranded DNA to fragments less than 1 kb in length in a total reaction volume of 80 µl in 1X Mung Bean Nuclease Reaction Buffer when incubated for 15 minutes at 37°C.

Reaction Conditions

1X Mung Bean Nuclease Reaction Buffer
Incubate at 30°C

1X Mung Bean Nuclease Reaction Buffer
30 mM NaCl
50 mM sodium acetate
1 mM ZnSO4
(pH 5 @ 25°C)

Storage Buffer

10 mM sodium acetate
0.1 mM Zinc Acetate
1 mM Cysteine
50% Glycerol
0.001% Triton® X-100
pH 5 @ 25°C

Molecular Weight

Theoretical: 39 kDa

Application Features

  • Removal of 3´ and 5´ extensions or single-stranded regions from DNA or RNA termini
  • Transcriptional mapping
  • Cleavage of hairpin loops
  • Excision of gene coding sequences from genomic DNA
  • Generation of new restriction sites

Product Notes

  1. It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn2+. The zinc acetate in the storage buffer fulfills the Zn2+ requirement of the enzyme even after dilution in a reaction.
  2. Do not attempt to heat inactivate, DNA will “breathe” before enzyme inactivates, causing undesirable degradation. 

References

  1. Kowalski, D. et al. (1976). Biochemistry. 15, 4457-4463.
  2. McCutchan, T.F. et al. (1984). Science. 225, 626-628.

Protocols, Manuals & Usage

Protocols

  1. Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210)?
  2. Is the Mung Bean Nuclease active in other NEBuffers?
  3. Can Mung Bean Nuclease be heat inactivated?
  4. Does Zn2+ need to be added to a Mung Bean Nuclease reaction?
  5. Will Mung Bean Nuclease degrade double stranded DNA?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective May 2, 2022, updated unit definition.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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