The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0249S     -20       RecA M0249SVIAL -20 1 x 0.1 ml 2 mg/ml   Rec A Reaction Buffer B0355SVIAL -20 1 x 1 ml 10 X
M0249L     -20       RecA M0249LVIAL -20 1 x 0.5 ml 2 mg/ml   Rec A Reaction Buffer B0355SVIAL -20 1 x 1 ml 10 X

Application Features

• Visualization of DNA structures with electron microscopy (3)
• D-loop mutagenesis (4)
• Screening libraries using RecA-coated probes (5,6)
• Cleavage of DNA at any single predetermined site (7,8,9)
• RecA mediated affinity capture for full length cDNA cloning (10, 11)

1. ATPγS which is required for triple helix formation is not supplied.
2. Heat Inactivation: 65°C for 20 min

1. West, S.C. (1992). Ann.Rev. Biochem.. 61, 603-640.
2. Zhumabayeva, B. et al. (1990). Biotechniques. 27, 834-845.
3. Zhumabayeva, B. et al. (2001). Biotechniques. 30, 512-520.
4. Pace, C.N. et al (1995). Protein Sci. 4, 2411-2423.
5. Radding, C.M. (1991). J. Biol.Chem.. 266, 5355-5358.
6. Wasserman, S.A. and Cozzarelli, N.R. (1985). Proc.Natl. Acad. Sci. USA. 82, 1079-1083.
7. Shortle, D. et al. (1980). Proc.Natl. Acad. Sci. USA. 77, 5375-5379.
8. Honigberg, S.M. et al. (1986). Proc.Natl. Acad. Sci. USA. 83, 9586-9590.
9. Rigas, B. et al. (1986). Proc.Natl. Acad. Sci. USA. 83, 9591-9595.
10. Ferrin, L.J. and Camerini-Otero, R.D. (1991). Science. 254, 1494-1497.
11. Koob, M. et al. (1992). Nucl.Acids Res.. 20, 5831-5836.
12. Koob, M. (1992). R. Wu(Ed.), Methodsin Enzymology. 216, 321-329. San Diego: Academic Press.

1. Using RecA and an oligonucleotide to form a stable triple helix

• DNA Damage and PreCR

1. What buffer should be used with RecA Protein?
2. Is magnesium required for triple strand formation using RecA Protein?
3. Is ATP gamma-S required for triple strand formation using RecA Protein?
4. Is the structure of the target DNA important when using RecA Protein?
5. How much RecA Protein should be added to the single stranded DNA to coat it?
6. What length single stranded oligo should be used with RecA Protein?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0249S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0249L_v1_10011090
• M0249L_v1_10021027
• M0249S_v1_10020722
• M0249S_v1_10031036
• M0249L_v1_10039475
• M0249S_v1_10053296
• M0249L_v1_10053294
• M0249S_v1_10065594
• M0249L_v1_10065596
• M0249L_v1_10084073
• M0249L_v1_10091334
• M0249S_v1_10091336
• M0249L_v1_10106700
• M0249S_v1_10128951
• M0249L_v1_10128953
• M0249S_v1_10149081
• M0249L_v1_10162401
• M0249L_v1_10169888
• M0249S_v1_10169891
• M0249S_v1_10211685
• M0249L_v1_10207557
• M0249S_L_v1_0291802
• M0249S_v1_10238475
• M0249S_v1_10252957
• M0249L_v1_10266623
• M0249S_v1_10268504
• M0249L_v1_10290193

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• RecA
• Rec A Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.