Nuclease BAL-31Product information
Progressive shortening of duplex DNA
Inactivation by treatment with EGTA
Supplied with 2X Reaction Buffer
Product SourcePurified from the culture medium of Alteromonas espejiana BAL-31. Contains a mixture of "fast" and "slow" species of the enzyme (3).
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Nuclease BAL-31 Reaction Buffer||-20||2X|
Advantages and Features
- Progressive shortening of double-stranded DNA fragments at both termini (4)
- Restriction site mapping (2)
Properties and Usage
Unit DefinitionOne unit is defined as the amount of enzyme required to remove 200 base pairs from each end of linearized double-stranded ΦX174 DNA (40 µg/ml) in a total reaction volume of 50 μl in 10 minutes at 30°C in 1X Nuclease BAL-31 Reaction Buffer.
1X Nuclease BAL-31 Reaction Buffer
Incubate at 30°C
1X Nuclease BAL-31 Reaction Buffer:
20 mM Tris-HCl
600 mM NaCl
12 mM MgCl2
12 mM CaCl2
1 mM EDTA
pH 8 @ 25°C
0.25 mM EDTA
10 mM Tris-HCl
50 mM NaCl
1.5 mM CaCl2
1.5 mM MgCl2
200 μg/ml BSA
pH 8.0 @ 25°C
Heat Inactivation65°C for 10 min
Quality Assurance Statement
- Purified free of detectable double-stranded endonuclease activity.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
- Nuclease BAL-31 Reaction Buffer
- Duplex products of the exonuclease are a mixture of blunt and staggered ends. This mixture can be cloned directly, although maximal ligation efficiency requires repairing the staggered ends with a suitable DNA polymerase.
- If necessary, the enzyme may be diluted in reaction buffer prior to use.
- Activity is linear with enzyme concentration.
- Heat Inactivation will only work in the presence of 20mM EGTA.
- Gray, H.B. et al. (1975). Nucl. Acids Res.. 2, 1459-1492.
- Legerski, R.J. et al. (1978). Nucl. Acids Res.. 5, 1445-1463.
- Wei, C.-F. et al. (1983). J. Biol. Chem.. 258, 13506-13512.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 5.73-5.75.
- Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment?
- Why does all of the DNA get degraded when I use Nuclease BAL-31?
- Can Nuclease BAL-31 be heat inactivated?
- Is Nuclease BAL-31 active in other NEBuffers?
- Can Nuclease BAL-31 treated DNA be cloned?
- What is a good control for the BAL-31 nuclease?
- Will Nuclease BAL-31 degrade RNA?
The supporting documents available for this product can be downloaded below.