Product Class: Kit

Luna® Cell Ready One-Step RT-qPCR Kit 


Catalog #E3030

Product Introduction

Go direct from cells to RNA quantitation without purification

  • Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (dye)
  • Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
  • Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
  • Features Luna WarmStart® RT paired with Hot Start Taq for increased thermostability and room temperature setup
  • Additional lysis preps available: Luna Cell Ready Lysis Module (NEB #E3032)
 

Protocols, Manuals & Usage

Protocols

  1. Protocol for Luna® Cell Ready One-Step RT-qPCR Kit Protocol (NEB #E3030)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. What cell lines are compatible with the Luna Cell Ready One-Step RT-qPCR Kit (NEB #E3030)?
  2. How many cells should I use for reliable detection?
  3. How many cells should I seed into cell culture plates to achieve optimal detection?
  4. Can cryopreserved cell lines or pelleted cells stored at -80°C be used with this kit? 
  5. How can lysis efficiency be determined?
  6. Why is it important to evaluate RNA damage or inhibition during cell lysis and one-step RT-qPCR detection?
  7. What is the “Luna Cell Ready RNA Protection Reagent” and when should it be used?
  8. Should I include a No-RT control reaction?
  9. Will the blue tracking dye interfere with detection?
  10. Is cell washing prior to lysis necessary?
  11. Which lysis format should I use?
  12. What protocol should be used for cell lysis? Can cells be lysed at room temperature or using a short protocol?
  13. When using cell lysates generated from Luna Cell Ready Lysis Module, can RT-qPCR experiments be set up at room temperature?
  14. How much cell lysate should I use as input? (NEB #3030)
  15. Can I use the cell lysates generated from the Luna Cell Ready Lysis Module for two-step RT-qPCR? 
  16. Why does the Luna Cell Ready One-Step RT-qPCR Kit contain more RT-qPCR reactions than lysis reactions?
  17. If either the Luna Cell Ready Lysis Module or Luna Universal One-Step RT-qPCR Kit runs out, can I order them separately?
  18. How can I obtain more technical help related to Luna Universal One-Step RT-qPCR Kit?

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