Product Class: Other

NEB® 10-beta Competent E. coli (High Efficiency)

Supplied with outgrowth medium optimized for NEB 10-beta & NEB Stable Competent E.coli ; please do not use SOC outgrowth medium.

Now available in 96-well plate format.

Product Introduction

NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.

  • High efficiency strain ideal for cloning large plasmids and BACs
  • Available in single-use vials, 200 μl vials, 96-well plate and electrocompetent format
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Catalog # Size Concentration
C3019H 20.0 x 0.05 ml
C3019I 6.0 x 0.2 ml

Featured Videos

View Video Library
  • TransformationTips_VideoThumb_282x210_0616

    Tips for Successful Transformations with NEB® Competent Cells

  • TransformationProtocol_VideoThumb_282x210_0616

    How to Perform a Transformation with NEB® Competent Cells

  • TransformationVideo_thumb

    The Mechanism of Transformation with Competent Cells

  • OverviewOfPCRCloning_720

    Overview of PCR Cloning

  • TraditionalCloningWorkflow_720

    Traditional Cloning Workflow

  • nebuilder_3primemismatchvideo_thumb

    NEBUILDER HIFI DNA ASSEMBLY® Removal of 3´ end Mismatches

  • GlassBeadsvideo_1920

    Fast transformation plating with glass beads

Protocols, Manuals & Usage

Protocols

  1. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (High Efficiency) (C3019H/C3019I)
  2. 5 Minute Transformation Protocol using NEB10-beta Competent E. coli  (C3019H/C3019I)
  3. High Efficiency Transformation Protocol with NEB 10-beta in 96-well Plate Format (NEB# C3019P)

Usage & Guidelines

Application Notes

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Which competent cell strains are compatible with Gateway® Cloning?
  2. What are the strain properties (C3019)?
  3. What is the difference between NEB #C3019H and NEB #C3019I?
  4. What is the shelf life for this strain (NEB #C3019H and NEB #C3019I)?
  5. Which strain of Competent E.coli should I use for general cloning?
  6. Does plasmid size affect transformation efficiency (C3019)?
  7. How should I calculate the transformation efficiency (C3019)?
  8. Can I store competent cells at -20°C instead of -80°C?
  9. What is the optimal heat shock time for this strain (NEB #C3019H and NEB #C3019I)?
  10. How long should I incubate cells on ice after DNA has been added (NEB #C3019H and NEB #C3019I)?
  11. Which kind of transformation tubes should be used?
  12. What volume of DNA can be added into competent cells?
  13. Are NEB's competent cells compatible with the “Mix & Go" protocol?
  14. What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix?
  15. Why did Synthetic Biologist Chris Voigt of MIT choose NEB 10-beta for DNA assembly and cloning?
  16. How should I store the NEB 10-beta/Stable Outgrowth Medium?
  17. How should fragments be prepared for assembly using NEBuilder HiFi?
  18. Can the 96-well plate format of NEB 10-beta Competent E.coli, NEB #C3019P, be separated into smaller sections?
  19. How does the transformation efficiency of the 96-well plate format (NEB #C3019P) compare to the other formats?

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