Product Class: Other

BL21 Competent E. coli

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Product Introduction

BL21 Competent E. coli is a widely used non-T7 expression E. coli strain and is suitable for transformation and protein expression. This strain does not express the T7 RNA Polymerase.

  • Ideal for Plac, Ptac, Ptrc ParaBAD expression vectors
  • Protease deficient
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Catalog # Size Concentration
C2530H 20.0 x 0.05 ml

Protocols, Manuals & Usage

Protocols

  1. Transformation Protocol (C2530)
  2. 5 Minute Transformation Protocol (C2530)
  3. Protocol for Protein Expression Using BL21 (C2530)
  4. Transformation Protocol (NEB #C2530)

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
  2. What are the solutions/recipes (C2530)?
  3. What are the strain properties (C2530)?
  4. Why are there no colonies or no growth in liquid culture (C2530)?
  5. Why is the induced protein insoluble (C2530)?
  6. Why is there no protein visible on gel or no activity (C2530)?
  7. Can I store competent cells at -20°C instead of -80°C?
  8. Which kind of transformation tubes should be used?
  9. What volume of DNA can be added into competent cells?
  10. What is the shelf life for this strain (NEB #C2350H)?
  11. Are NEB's competent cells compatible with the “Mix & Go" protocol?
  12. What is the difference between BL21 and BL21(DE3) competent E.coli cells?
  13. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?

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