BL21 Competent E. coliProduct information
|20 x 0.05 ml||-||Unavailable in your region|
- Transformation efficiency: 1–5 x 107 cfu/μg pUC19 DNA
- Routine non-T7 expression
- Deficient in proteases Lon and OmpT
- Resistant to phage T1 (fhuA2)
- B Strain
- Free of animal products
- Routine non-T7 expression
GenotypefhuA2 [lon] ompT gal [dcm] ΔhsdS
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|pUC19 Vector||-20||0.05 ng/μl|
|SOC Outgrowth Medium||4||1X|
Properties and Usage
|Antibiotics for Plasmid Selection||Working Concentration|
- Ships on dry ice
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
- Transformation Efficiency:
The competent cells are tested for transformation efficiency and pass minimum release criteria. Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 μg of plasmid into a given volume of competent cells.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
BL21 Competent E. coli
SOC Outgrowth Medium
- CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
- STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
- COMPATIBLE EXPRESSION VECTORS/PROMOTERS: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T5-lacO
Note: Expression from vectors containing a T5-lacO promoter will not be regulated unless the expression vector encodes the lacI gene or a compatible vector expresses LacI or the host strain overproduces LacI (e.g NEB #C3037 NEB Express Iq Competent E. coli)."
- Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
- What are the solutions/recipes (C2530)?
- What are the strain properties (C2530)?
- Why are there no colonies or no growth in liquid culture (C2530)?
- Why is the induced protein insoluble (C2530)?
- Why is there no protein visible on gel or no activity (C2530)?
- Can I store competent cells at -20°C instead of -80°C?
- Which kind of transformation tubes should be used?
- What volume of DNA can be added into competent cells?
- What is the shelf life for this strain (NEB #C2350H)?
- Are NEB's competent cells compatible with the “Mix & Go" protocol?
- What is the difference between BL21 and BL21(DE3) competent E.coli cells?
- How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?