Product Class: Reaction Buffer

OneTaq® GC Reaction Buffer Pack

This buffer is still available as a companion product with M0480 and M0481.
Catalog #SizeConcentration
B9023S8 (4 x 2.0ml)5X


5X OneTaq GC Reaction Buffer and OneTaq High GC Enhancer are optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer and supplemental enhancer have been formulated for robust yields of difficult or GC-rich amplicons. The OneTaq GC Reaction Buffer is recommended for GC-rich (>65% GC) amplicons. For particularly difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions with OneTaq GC Reaction Buffer. 

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
OneTaq High GC Enhancer50%
OneTaq® GC Reaction Buffer Pack-205X

Properties and Usage

Storage Temperature


1X Buffer Components

1X Buffer Components

80mM Tris-SO4
20mM (NH4)2SO4
2mM MgSO4
5% Glycerol
0.06% IGEPAL® CA-630
0.05% Tween® 20
pH 9.2@25°C

Quality Control

Quality Assurance Statement

  • The reaction buffer and supplement are free of detectable nucleases.

Supporting Documents

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.


  1. OneTaq High GC Enhancer:
    10 mM Tris-HCl
    25% DMSO
    25% Glycerol
    pH 9.2 @ 25°C

  1. The buffer arrived thawed.  Are the buffer and enzyme still active?
  2. Which buffer should I use if I want to control the level of magnesium in the reaction?