Product Class: Diluent Buffer

Diluent C

Catalog #SizeConcentration
B8003S5 ml1X


Diluent C is recommended for making dilutions of New England Biolabs, Inc. restriction enzymes.

When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Enzymes should be stored at their selling concentrations.

Each vial contains 1.3 ml of diluent buffer.

Properties and Usage

Storage Temperature


1X Buffer Components

1X Buffer Components

250mM NaCl
10mM Tris-HCl
0.1mM EDTA
0.15% Triton® X-100
200μg/ml BSA
50% Glycerol
pH 7.4@25°C

Supporting Documents

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.


  1. These enzyme diluent buffers are not for use with VentR® DNA Polymerases. For diluting VentR® DNA Polymerases, use (NEB #B8004S).
  1. What is supplied with the Diluent Buffer C?
  2. What is the composition of the Diluent Buffer C?
  3. How should the Diluent Buffer be used?
  4. How long can the diluted enzyme be stored?
  5. Why is my Restriction Enzyme not cutting DNA?
  6. Why do I see additional DNA bands on my gel after a restriction digest?
  7. Why do I see a DNA smear on an agarose gel after a restriction digest?
  8. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?