Product Class: Reaction Buffer

Antarctic Phosphatase Reaction Buffer

Catalog #SizeConcentration
B0289S6 ml10X


At a 1X concentration this reaction buffer assures optimal activity of the enzyme.

Properties and Usage

Storage Temperature


1X Buffer Components

1X Buffer Components

50mM Bis-Tris-Propane-HCl
1mM MgCl2
0.1mM ZnCl2
pH 6.0@25°C

Quality Control

Quality Assurance Statement

  • The reaction buffer is free of detectable nucleases.

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

Supporting Documents

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.