Chaps Cell Extract Buffer (10X)Product information
|5 ml||-||Unavailable in your region|
Chaps Cell Extract Buffer (10X) #9852
|9852S||5 ml||---||In Stock||---|
|9852||carrier free and custom formulation / quantity||email request|
|Chaps Cell Extract Buffer (10X)||5 ml|
|30X Reducing Agent (1.25 M DTT)||250 µl|
Storage: Supplied as a 10X solution. Store at -20°C
Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommended for the preparation of cytoplasmic cell lysates to be used with our caspase signaling pathway antibodies (see Companion Products).
Directions for Use
Treat cells by adding fresh media containing regulator for desired time.
Aspirate media from cultures; wash cells three times with PBS; aspirate. Scrape cells into PBS and spin down to pellet.
Lyse cells by adding Chaps Cell Extract Buffer (1 volume of cell pellet). Resuspend cells in the buffer, freeze and thaw three times, centrifuge lysate at 14,000 rpm. Keep the supernatant and discard the pelleted cell debris.
Add SDS Sample Buffer and heat sample to 95–100°C for 5 minutes; cool on ice.
Microcentrifuge for 5 minutes.
Load 5 µL onto SDS-PAGE gel (10 cm x 10 cm).
Solutions and Reagents
CHAPS Cell Extract Buffer (5mL) 1X concentration: 50 mM Pipes/HCl (pH 6.5), 2 mM EDTA, 0.1% Chaps, 20 µg/ml Leupeptin, 10 µg/ml Pepstatin A,10 µg/ml Aprotinin. Add DTT to 5mM.
1.25 M (250X) DTT (0.25 mL).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.