Phospho-(Ser/Thr) Phe AntibodyProduct information
Phospho-(Ser/Thr) Phe Antibody
|100 µl (10 western blots)||-||Unavailable in your region|
Product Pathways - Motif Antibodies
Phospho-(Ser/Thr) Phe Antibody #9631
|9631S||100 µl (10 western blots)||---||In Stock||---|
|9631||carrier free and custom formulation / quantity||email request|
|W||1:1000||All Species Expected||Endogenous||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA)
Specificity / Sensitivity
Phospho-(Ser/Thr) Phe Antibody detects phospho-serine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. The antibody does not cross-react with the nonphosphorylated form of these motifs, nor does it cross-react with other phospho-serine/threonine-containing proteins and peptides. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-serine/threonine-phenylalanine-containing peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) Phe Antibody.
A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins such as 14-3-3 (2). These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. CST has pioneered the development of phospho-motif specific antibodies, which are invaluable tools for probing the complexity of phospho-regulatory pathways.
Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, a kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine (3). RSK1, p70S6K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity (4,5). The Phospho-(Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.
- Kalabis, J. et al. (2006) J Biol Chem 281, 6434-41. Applications: IP, Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 9606 Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb
- 9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody
- 2261 Phospho-(Ser) PKC Substrate Antibody
- 9611 Phospho-(Ser/Thr) Akt Substrate Antibody
- 9621 Phospho-(Ser/Thr) PKA Substrate Antibody
- 9614 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb
- 9624 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
- 9381 Phospho-Threonine Antibody (P-Thr-Polyclonal)
- 9391 Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)
- 2351 Phospho-Threonine-X-Arginine Antibody
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 6314 SignalSilence® PDK1 siRNA I
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.