MDM2 (D1V2Z) Rabbit mAbProduct information
|100 µl (10 western blots)||-||Unavailable in your region|
Product Pathways - DNA Damage
MDM2 (D1V2Z) Rabbit mAb #86934
|86934S||100 µl (10 western blots)||---||In Stock||---|
|86934||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
MDM2 (D1V2Z) Rabbit mAb recognizes endogenous levels of total MDM2 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect full-length MDM2, as well as isoforms A, 2-C, alpha, 2-F, 2-G, and 11. This antibody may detect isoform A1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val280 of human MDM2 protein.
Western blot analysis of SJSA-1, U-2 OS, and Saos-2 cells, untreated (-) or treated with Nutlin 3a (10 μM, 24 hr; +), using MDM2 (D1V2Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).
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