Keratin 18 (DC10) Mouse mAbProduct information
Product Pathways - Cytoskeletal Signaling
Keratin 18 (DC10) Mouse mAb #4548
|4548S||100 µl||---||In Stock||---|
|4548T||20 µl||---||In Stock||---|
|4548||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Keratin 18 (DC10) Mouse mAb detects endogenous levels of total keratin 18 protein. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody (isotype: IgG1) is produced by immunizing mice with human PMC-42 breast carcinoma cells.
Western blot analysis of extracts from HeLa and A431 cells, using Keratin 18 (DC10) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using Keratin 18 (DC10) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Keratin 18 (DC10) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human benign prostate hyperplasia, using Keratin 18 (DC10) Mouse mAb.
Confocal immunofluorescent analysis of HepG2 (left) and SH-SY5Y (right) cells using Keratin 18 (DC10) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of HeLa cells, using Keratin 18 (DC10) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 4546 Keratin 8/18 (C51) Mouse mAb
- 5425 Normal Goat Serum
- 4545 Pan-Keratin (C11) Mouse mAb
- 4523 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 4528 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 7072 Phototope-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 8112 SignalStain® Antibody Diluent
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.