SUZ12 (D39F6) XP® Rabbit mAbProduct information
Product Pathways - Chromatin Regulation / Epigenetics
SUZ12 (D39F6) XP® Rabbit mAb #3737
|3737S||100 µl (10 western blots)||---||In Stock||---|
|3737T||20 µl (2 western blots)||---||In Stock||---|
|3737||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||83||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq
Species predicted to react based on 100% sequence homology: Pig, Horse.
Directions For Use
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Specificity / Sensitivity
SUZ12 (D39F6) XP® Rabbit mAb detects endogenous levels of SUZ12 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of the human SUZ12 protein.
Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells (left) and NTERA2 cells (right) using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Flow cytometric analysis of HeLa cells using SUZ12 (D39F6) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP® Rabbit mAb or RING1B (D22F2) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. SUZ12 and RING1B are known to associate with each other on chromatin. The figure shows binding of both SUZ12 and RING1B across HOXD genes, which are known target genes of both SUZ12 and RING1B (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).
The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (5). Suppressor of Zeste 12 (SUZ12) is a component of the PRC2 complex, which together with Ezh2 and Eed is absolutely required for histone methyl-transferase activity (6). SUZ12 contains a C2H2 zinc finger domain similar to the zinc finger domains found in sequence-specific DNA binding proteins and may mediate the interaction between EZH2 and nucleosomes (6). SUZ12 is overexpressed in several human tumors, including tumors of the colon, breast and liver (7,8).
- Boyer, L.A. et al. (2006) Nature 441, 349-53.
- Lee, T.I. et al. (2006) Cell 125, 301-13.
- Cao, R. et al. (2002) Science 298, 1039-43.
- Müller, J. et al. (2002) Cell 111, 197-208.
- Wang, H. et al. (2004) Nature 431, 873-8.
- Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
- Kirmizis, A. et al. (2003) Mol Cancer Ther 2, 113-21.
- Kirmizis, A. et al. (2004) Genes Dev 18, 1592-605.
- Pasini, D. et al. (2010) Nucleic Acids Res 38, 4958-69. Applications: Chromatin IP.
- Schmitz, S.U. et al. (2011) EMBO J 30, 4586-600. Applications: Chromatin IP, Western Blotting.
- Pasini, D. et al. (2010) Nature 464, 306-10. Applications: Chromatin IP.
- Chan, K.M. et al. (2013) Genes Dev 27, 985-90. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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