Phospho-IRS-1 (Tyr1222) AntibodyProduct information
Phospho-IRS-1 (Tyr1222) Antibody
|100 µl (10 western blots)||
Product Pathways - Metabolism
Phospho-IRS-1 (Tyr1222) Antibody #3066
|3066S||100 µl (10 western blots)||---||In Stock||---|
|3066||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Human, Mouse.
Specificity / Sensitivity
Phospho-IRS-1 (Tyr1222) Antibody detects transfected levels of IRS-1 only when phosphorylated at Tyr1222. The antibody may cross-react with other activated receptor tyrosine kinases (RTKs) and docking proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1222 of human IRS-1. Antibodies are purified by protein A and peptide affinity chromatography.
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).
Phosphorylation of tyrosine 1222 of IRS-1 was identified in insulin stimulated cells (11). Phosphorylated Tyr1222 provides a docking site for the SH2 domain of PTP2C, which may mediate dephosphorylation of IRS-1 and lead to negative feedback of insulin signaling (12).
- Sun, X.J. et al. (1991) Nature 352, 73-77.
- Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
- Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
- Wang, L.M. et al. (1993) Science 261, 1591-1594.
- Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
- Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
- Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
- Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
- De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
- Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
- Sun, X.J. et al. (1993) Mol. Cell. Biol. 13, 7418-7428.
- Rocchi, S. et al. (1995) Endocrinology 136, 5291-5297.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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