Product Class: Restriction Endonuclease

XhoI
neb31 cloned at NEB recombinant time saver dil_A 37 65 Heat CpG

XhoI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10161948. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.


NEB restriction endonuclease that recognizes the sequence C^TCGA_G
Isoschizomers | Single Letter Code

Product Introduction

Catalog # Size Concentration
R0146S 5000.0 units 20000 units/ml
R0146L 25000.0 units 20000 units/ml
R0146M 25000.0 units 100000 units/ml

Featured Videos

View Video Library
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    Reduce Star Activity with High-Fidelity Restriction Enzymes

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    NEB® TV Ep. 15 – Applications of Restriction Enzymes

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    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

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    Restriction Enzyme Digest Problem: Too Many DNA Bands

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    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
  3. Restriction Digest Protocol
  4. Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
  5. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Why isn't XhoI cutting?
  2. Does XhoI produce commonly used compatible ends?
  3. How does XhoI differ from its isoschizomer, PaeR71?
  4. What is the molecular weight of XhoI?
  5. What is the activity of XhoI at 25°C?
  6. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  7. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  8. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  9. Does NEB offer any BSA-free and/or animal origin-free restriction enzymes for linearization of plasmids for mRNA vaccine development?
  10. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
  11. Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?

Troubleshooting

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