Product Class: Restriction Endonuclease

NdeI
neb31 cloned at NEB recombinant time saver dil_A 37 65 Heat

NdeI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10133989. Learn more.We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence CA^TA_TG
Isoschizomers | Single Letter Code

Product Introduction

Catalog # Size Concentration
R0111S 4000.0 units 20000 units/ml
R0111L 20000.0 units 20000 units/ml

Featured Videos

View Video Library
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    Reduce Star Activity with High-Fidelity Restriction Enzymes

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    TIME-SAVER™ Protocol for Restriction Enzyme Digests

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    NEB® TV Ep. 15 – Applications of Restriction Enzymes

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    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

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    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

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    Why is My Restriction Enzyme Not Cutting DNA?

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    Restriction Enzyme Digest Problem: Too Many DNA Bands

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    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is NdeI a Time-Saver™ Qualified enzyme?
  2. Is NdeI activity sensitive to dam, dcm or mammalian CpG methylation?
  3. Does NdeI have any isoschizomers?
  4. NdeI seems to be digesting my DNA correctly, but when I try to ligate it, I obtain no colonies. Is there a potential explanation for what I am observing?
  5. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  6. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

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