Product Class: Other

RNase H
NEBU recombinant 37 65 Heat

Product Introduction

RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA.

  • Recombinant enzyme supplied with Reaction Buffer
  • Tested for the absence of endonucleases, ss-DNase and other RNases
  • This enzyme does not digest single or double-stranded DNA
  • Thermostable RNase H (NEB #M0523) is available for higher temperature reactions
  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.
Catalog # Size Concentration
M0297S 250.0 units 5000 units/ml
M0297L 1250.0 units 5000 units/ml

Product Information

Description

RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.

Product Source

An E. coli strain that carries the cloned RNase H gene (rnh) from Escherichia coli.
This product is related to the following categories:
RNases,
cDNA Synthesis & Reverse Transcriptases Products,
This product can be used in the following applications:
RT-PCR & cDNA Synthesis,
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • M0297S     -20    
      RNase H M0297SVIAL -20 1 x 0.05 ml 5,000 units/ml
      RNase H Reaction Buffer B0297SVIAL -20 1 x 1.5 ml 10 X
  • M0297L     -20    
      RNase H M0297LVIAL -20 1 x 0.25 ml 5,000 units/ml
      RNase H Reaction Buffer B0297SVIAL -20 1 x 1.5 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 37°C.

Reaction Conditions

1X RNase H Reaction Buffer
Incubate at 37°C

1X RNase H Reaction Buffer
50 mM Tris-HCl
75 mM KCl
3 mM MgCl2
10 mM DTT
(pH 8.3 @ 25°C)

Storage Buffer

50 mM KCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 minutes

References

  1. Davis, R. et al. (1988). Cell Biol. 8, 4745-4755.
  2. Gubbler, U. and Hoffman, B.J. (1983). Gene. 25, 263-269.
  3. Donnis-Keller, H. (1979). Nucl. Acids Res. 7, 179.
  4. Goodwin, E.C. and Rottman, F.M. (1992). Nucl. Acids Res. 20, 916.

Protocols, Manuals & Usage

Protocols

  1. Cleavage of an RNA strand in an RNA-DNA hybrid with RNase H (NEB #M0297)

FAQs & Troubleshooting

FAQs

  1. When I thaw the 10X RNase H Reaction Buffer, I see a white precipitate.  What is it and what should I do about it?
  2. Is DNA Polymerase I active in RNase H buffer?
  3. Which side of the RNA base does RNase H cut?
  4. What is the activity of RNase H at 30°C?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.