Product Class: Other

T4 Polynucleotide Kinase
NEBU cloned at NEB recombinant 37 65 Heat

Product Introduction

  • 5' phosphorylation of DNA/RNA for subsequent ligation
  • End labeling DNA or RNA for probes and DNA sequencing
  • Removal of 3' phosphoryl groups
 
Catalog # Size Concentration
M0201S 500.0 units 10000 units/ml
M0201L 2500.0 units 10000 units/ml

Featured Videos

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    DNA Blunting Tutorial

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    The Mechanism of DNA Phosphorylation

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    NEBioCalculator® - Using the ds: mass -> ends module to plan a phosphorylation reaction using T4 Polynucleotide Kinase (T4 PNK)

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Protocols, Manuals & Usage

Protocols

  1. Radioactive Labeling with T4 PNK or T4 PNK (3´ phosphatase minus)
  2. Non-radioactive Phosphorylation with T4 PNK or T4 PNK (3´ phosphatase minus)
  3. End-labeling Protocol

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What factors can cause incomplete phosphorylation when using T4 Polynucleotide Kinase?
  2. Can I use T4 Polynucleotide Kinase and T4 DNA Ligase in the same reaction buffer?
  3. How can the rate of phosphorylation be improved when using T4 Polynucleotide Kinase?
  4. Do I need to dephosphorylate prior to labeling?
  5. How much substrate can be phosphorylated in a standard reaction?
  6. How many units of T4 Polynucleotide Kinase should be used for a typical reaction?
  7. How do I inactivate the enzyme?
  8. How to calculate the molarity of ends?
  9. What labels can be used?
  10. Will T4 Polynucleotide Kinase work in rCutSmart Buffer?

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